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古老病毒入侵
作者:USMedEdu
发表时间:2010-09-17
更新时间:2010-09-17
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古老病毒通过入侵重塑人类基因组

译者:Docofsoul

http://news.dxy.cn/bbs/topic/18288941


《每日科学》2010年9月13日报道 —— 新加坡基因组研究院(GIS, 隶属于新加坡科技研究局(A*STAR)的生物医学研究院)的科学家以及来自新加坡国立大学、新加坡南洋理工大学、杜克-新加坡大学医学研究院与普林斯顿大学的同事们最近发现:数百万年前“入侵”人类基因组的病毒已经改变了人类胚胎干细胞(ES细胞)中的基因开启与关闭方式。



科学家已经发现数百万年前“入侵”人类基因组的病毒已经改变了人类胚胎干细胞(ES细胞)中的基因开启与关闭方式。(照片来源:iStockphoto/Martin McCarthy)

这一研究为生理学与医学诺贝尔奖获得者芭芭拉•麦克林托克(Barbara McClintock)于上世纪五十年代提出的理论提供了明确的证据。芭芭拉•麦克林托克的理论推测:转座因子,即可移动的遗传物质(DNA)片段(比如说病毒序列),一旦插入基因组,就能成为影响基因调节的“控制因子”。

本发现对于推进干细胞研究进程、增强干细胞研究为再生医学效劳的潜力都算得上是重要贡献。 由新加坡基因组研究院精英小组负责人吉拉姆•布尔克(Guillaume Bourque)博士率队领导了本研究。本研究的论文发表于2010年6月6日的《Nature Genetics》(《自然•遗传学》)。

通过运用新的测序技术,科学家们研究了人类与小鼠胚胎干细胞(ES细胞)中三种调节蛋白质(OCT4、NANOG 与 CTCF) 的染色体组定位(基因组定位)。令人感兴趣的是,在科学家发现大量的相似点的同时,他们也发现了在人类中受到调控的基因方式与基因类型的许多不同点。尤其是,他们发现:数百万年前自行插入人类基因组的特定类型病毒已经戏剧性地改变了人类干细胞基因调控网络。

德克萨斯州大学阿灵顿分校副教授Cedric Feschotte 博士说:“本研究是计算与实验双管齐下的代表作,提供了无可置疑的全新的证据:一些经常被斥责为纯粹垃圾DNA的转座因子,恰恰正是人类发育调控密码的关键成分。”

在基因调控网络的研究中,人类模型系统与小鼠模型系统之间的比较研究有助于增进对干细胞分化成体内不同细胞类型的具体过程的理解。布尔克博士说:“这种理解在促使再生医学的百尺竿头更进一步地发展 —— 从而解决诸如帕金森病与白血病等问题方面是至关重要的。除了在本研究中利用基因调控网络中的小鼠胚胎干细胞的优势外, 深入研究必须更加直接地集中于人类干细胞。这是因为将某一种类上完成的研究成果转向对另一种类的研究上时必然会遇上的挑战。为了让干细胞方面的发现能够用于临床实践,在人类与(非人类的)灵长类干细胞两个方面还有更多的研究工作需要完成。”

加利福尼亚州立大学神经学Rudi Schmid 特聘教授、哲学博士雷蒙德•怀特(Raymond L. White)教授说:“本论文报告了令人非常激动的新发现,证实了一个全新的、迥然不同的基因表达的调控机制。通过将小鼠的基因组与人类基因组的直接比较,科学家能够显示:在两种种类之间,基因调控因子的结合点经常不在同一位置。这本身就足够令人惊讶的了,但是研究者作了进一步的探索,证实许多位点都嵌合在称之为‘转位’因子的一类DNA序列中,这是因为他们具有在基因组中移动到新的位置的能力。存在很多这样的相信是病毒基因组进化残余部分的因子,但我们所了解到的(信息中)还有着非常出人意外的情形:它们到达新的(基因组)位置时,还携带着调控因子结合位点。这些在调控方面的变化估计可能在携带它们的有机体上产生重大变化。确实,许多学者相信调控方面的变化处于物种形成的核心,可能在人从其祖先的进化历程中扮演了一个重要角色。本论文可能成为这一研究领域的里程碑式的论文。”

美国能源部联合基因组研究所所长、劳伦期•伯克利国家实验室伯克利实验分室基因组学部主任埃迪•拉宾(Eddy Rubin)博士补充说:“这个运用了比较基因组学策略的研究在人类胚胎干细胞(ES细胞)中发现了重要的人类特异性属性。该论文所提供的信息意义重大,应该有助于推进再生医学领域的发展,相信会有不俗的积极表现。”

参考文献:

Galih Kunarso, Na-Yu Chia, Justin Jeyakani, Catalina Hwang, Xinyi Lu, Yun-Shen Chan, Huck-Hui Ng, Guillaume Bourque. Transposable elements have rewired the core regulatory network of human embryonic stem cells.Nature Genetics, 2010; 42 (7): 631 DOI: 10.1038/ng.600

(《转位因子重新连接人类胚胎干细胞的核心调控网络》)

(Docofsoul 译于2010-09-14)






导读:六十年前, 生理学与医学诺贝尔奖获得者芭芭拉•麦克林托克提出了在当时可谓石破天惊的理论推测:转座因子,即可移动的遗传物质(DNA)片段(比如说病毒序列),一旦插入基因组,就能成为影响基因调节的“控制因子”。六十年后,科学家们凭借全新的测序技术,通过电脑计算与实验设计双管齐下的非凡策略,终于印证了这一假说。这一成就对基因组学的发展以及再生医学临床化都将有积极的意义。

—— Docofsoul


Ancient Viral Invasion Shaped Human Genome


ScienceDaily (Sep. 13, 2010) — Scientists at the Genome Institute of Singapore (GIS), a biomedical research institute of the Agency for Science, Technology and Research (A*STAR), and their colleagues from the National University of Singapore, Nanyang Technological University, Duke-NUS Graduate Medical School and Princeton University have recently discovered that viruses that 'invaded' the human genome millions of years ago have changed the way genes get turned on and off in human embryonic stem (ES) cells.





Scientists have discovered that viruses that "invaded" the human genome millions of years ago have changed the way genes get turned on and off in human embryonic stem cells. (Credit: iStockphoto/Martin McCarthy)


The study provides definitive proof of a theory that was first proposed in the 1950s by Nobel Laureate in physiology and medicine, Barbara McClintock, who hypothesized that transposable elements, mobile pieces of the genetic material (DNA), such as viral sequences, could be "control elements" that affect gene regulation once inserted in the genome.
This finding is an important contribution to the advancement of stem cell research and to its potential for regenerative medicine. Led by GIS Senior Group Leader Dr Guillaume Bourque, the study was published in Nature Genetics on June 6, 2010.
Through the use of new sequencing technologies, the scientists studied the genomic locations of three regulatory proteins (OCT4, NANOG and CTCF) in human and mouse embryonic stem (ES) cells. Interestingly, while the scientists found a lot of similarities, they also found many differences in the methods and the types of genes that are being regulated in humans. In particular, it was discovered that specific types of viruses that inserted themselves in the human genomes millions of years ago have dramatically changed the gene regulatory network in human stem cells.

"This study is a computational and experimental tour de force. It provides undeniable evidence that some transposable elements, which are too often dismissed as merely junk DNA, are key components of a regulatory code underlying human development," said Dr Cedric Feschotte, Associate Professor of the University of Texas Arlington.

The comparisons between the human and mouse model system in the study of gene regulatory networks help to advance the understanding of how stem cells differentiate into various cell types of the body. "This understanding is crucial in the improved development of regenerative medicine for diseases such as Parkinson's disease and leukaemia," said Dr Bourque. "Despite the advantages of using mouse ES cells in the study of gene regulatory networks, further research must focus more directly on human stem cells. This is due to the inherent challenges of converting the results of studies done from one species to that of the next. More research will need to be done in both human and non-human primate stem cells for findings on stem cells to be used in clinical application."
Prof Raymond L. White, PhD, Rudi Schmid Distinguished Professor of Neurology, University of California said, "The paper reports very exciting new findings that establish a new and fundamentally distinct mechanism for the regulation of gene expression. By comparing the genomes of mouse with human, the scientists were able to show that the binding sites for gene regulatory factors are very often not in the same place between the two species. This by itself would be very surprising, but the investigators go further and demonstrate that many of the sites are imbedded within a class of DNA sequences called "transposable" elements because of their ability to move to new places in the genome. There are a number of such elements believed to be the evolutionary remnants of viral genomes, but it was very surprising to learn that they were carrying binding sites for regulatory elements to new locations. These changes in regulation would be expected to create major changes in the organisms which carry them. Indeed, many think that regulatory changes are at the heart of speciation and may have played a large role in the evolution of humans from their predecessors. This is likely to be a landmark paper in the field."

Dr Eddy Rubin, Director of the U.S. Department of Energy Joint Genome Institute and Director of the Genomics Division at Lawrence Berkeley National Laboratory in Berkeley added, "This study using a comparative genomics strategy discovered important human specific properties of the regulatory network in human ES cells. This information is significant and should contribute to helping move the regenerative medicine field forward."

http://www.sciencedaily.com/releases/2010/06/100607101652.htm
----------------------------------------



Ancient Viral Invasion Shaped Human Genome

古老病毒通过入侵重塑人类基因组

译者:Docofsoul


ScienceDaily (Sep. 13, 2010) — Scientists at the Genome Institute of Singapore (GIS), a biomedical research institute of the Agency for Science, Technology and Research (A*STAR), and their colleagues from the National University of Singapore, Nanyang Technological University, Duke-NUS Graduate Medical School and Princeton University have recently discovered that viruses that 'invaded' the human genome millions of years ago have changed the way genes get turned on and off in human embryonic stem (ES) cells.

《每日科学》2010年9月13日报道 —— 新加坡基因组研究院(GIS, 隶属于新加坡科技研究局(A*STAR)的生物医学研究院)的科学家以及来自新加坡国立大学、新加坡南洋理工大学、杜克-新加坡大学医学研究院与普林斯顿大学的同事们最近发现:数百万年前“入侵”人类基因组的病毒已经改变了人类胚胎干细胞(ES细胞)中的基因开启与关闭方式。






Scientists have discovered that viruses that "invaded" the human genome millions of years ago have changed the way genes get turned on and off in human embryonic stem cells. (Credit: iStockphoto/Martin McCarthy)

科学家已经发现数百万年前“入侵”人类基因组的病毒已经改变了人类胚胎干细胞(ES细胞)中的基因开启与关闭方式。(照片来源:iStockphoto/Martin McCarthy)

The study provides definitive proof of a theory that was first proposed in the 1950s by Nobel Laureate in physiology and medicine, Barbara McClintock, who hypothesized that transposable elements, mobile pieces of the genetic material (DNA), such as viral sequences, could be "control elements" that affect gene regulation once inserted in the genome.

这一研究为生理学与医学诺贝尔奖获得者芭芭拉•麦克林托克(Barbara McClintock)于上世纪五十年代提出的理论提供了明确的证据。芭芭拉•麦克林托克的理论推测:转座因子,即可移动的遗传物质(DNA)片段(比如说病毒序列),一旦插入基因组,就能成为影响基因调节的“控制因子”。

This finding is an important contribution to the advancement of stem cell research and to its potential for regenerative medicine. Led by GIS Senior Group Leader Dr Guillaume Bourque, the study was published in Nature Genetics on June 6, 2010.

本发现对于推进干细胞研究进程、增强干细胞研究为再生医学效劳的潜力都算得上是重要贡献。 由新加坡基因组研究院精英小组负责人吉拉姆•布尔克(Guillaume Bourque)博士率队领导了本研究。本研究的论文发表于2010年6月6日的《Nature Genetics》(《自然•遗传学》)。

Through the use of new sequencing technologies, the scientists studied the genomic locations of three regulatory proteins (OCT4, NANOG and CTCF) in human and mouse embryonic stem (ES) cells. Interestingly, while the scientists found a lot of similarities, they also found many differences in the methods and the types of genes that are being regulated in humans. In particular, it was discovered that specific types of viruses that inserted themselves in the human genomes millions of years ago have dramatically changed the gene regulatory network in human stem cells.

通过运用新的测序技术,科学家们研究了人类与小鼠胚胎干细胞(ES细胞)中三种调节蛋白质(OCT4、NANOG 与 CTCF) 的染色体组定位(基因组定位)。令人感兴趣的是,在科学家发现大量的相似点的同时,他们也发现了在人类中受到调控的基因方式与基因类型的许多不同点。尤其是,他们发现:数百万年前自行插入人类基因组的特定类型病毒已经戏剧性地改变了人类干细胞基因调控网络。

"This study is a computational and experimental tour de force. It provides undeniable evidence that some transposable elements, which are too often dismissed as merely junk DNA, are key components of a regulatory code underlying human development," said Dr Cedric Feschotte, Associate Professor of the University of Texas Arlington.

德克萨斯州大学阿灵顿分校副教授Cedric Feschotte 博士说:“本研究是计算与实验双管齐下的代表作,提供了无可置疑的证据:一些经常被斥责为纯粹垃圾DNA的转座因子,恰恰正是人类发育调控密码的关键成分。”

The comparisons between the human and mouse model system in the study of gene regulatory networks help to advance the understanding of how stem cells differentiate into various cell types of the body. "This understanding is crucial in the improved development of regenerative medicine for diseases such as Parkinson's disease and leukaemia," said Dr Bourque. "Despite the advantages of using mouse ES cells in the study of gene regulatory networks, further research must focus more directly on human stem cells. This is due to the inherent challenges of converting the results of studies done from one species to that of the next. More research will need to be done in both human and non-human primate stem cells for findings on stem cells to be used in clinical application."

在基因调控网络的研究中,人类模型系统与小鼠模型系统间的比较研究有助于增进对干细胞分化成体内不同细胞类型的具体过程的理解。布尔克博士说:“这种理解在促使再生医学的百尺竿头更进一步地发展—— 从而解决诸如帕金森病与白血病等问题方面是至关重要的。除了在本研究中利用基因调控网络中的小鼠胚胎干细胞的优势外, 深入研究必须更加直接地集中于人类干细胞。这是因为将某一种类上完成的研究成果转向对另一种类的研究上时必然会遇上的挑战。为了让干细胞方面的发现能够用于临床,在人类与(非人类的)灵长类干细胞两个方面还有更多的研究工作需要完成。”

Prof Raymond L. White, PhD, Rudi Schmid Distinguished Professor of Neurology, University of California said, "The paper reports very exciting new findings that establish a new and fundamentally distinct mechanism for the regulation of gene expression. By comparing the genomes of mouse with human, the scientists were able to show that the binding sites for gene regulatory factors are very often not in the same place between the two species. This by itself would be very surprising, but the investigators go further and demonstrate that many of the sites are imbedded within a class of DNA sequences called "transposable" elements because of their ability to move to new places in the genome.


是加利福尼亚州立大学神经学Rudi Schmid 特聘教授、哲学博士雷蒙德•怀特(Raymond L. White)教授说:“本论文报告了令人非常激动的新发现,证实了一个全新的、迥然不同的基因表达的调控机制。通过将小鼠的基因组与人类基因组的直接比较,科学家能够显示:在两种种类之间,基因调控因子的结合点经常不在同一位置。这本身就足够令人惊讶的了,但是研究者作了进一步的探索,证实许多位点都嵌合在称之为‘转位’因子的一类DNA序列中,这是因为他们具有在基因组中移动到新的位置的能力。”


There are a number of such elements believed to be the evolutionary remnants of viral genomes, but it was very surprising to learn that they were carrying binding sites for regulatory elements to new locations. These changes in regulation would be expected to create major changes in the organisms which carry them. Indeed, many think that regulatory changes are at the heart of speciation and may have played a large role in the evolution of humans from their predecessors. This is likely to be a landmark paper in the field."

存在很多这样的相信是病毒基因组进化残余部分的因子,但我们所了解到的(信息中)还有着非常出人意外的情形:它们到达新的(基因组)位置时,还携带着调控因子结合位点。这些在调控方面的变化估计可能在携带它们的有机体上产生重大变化。确实,许多人相信调控方面的变化处于物种形成的核心,可能在人从其祖先的进化历程中扮演了一个重要角色。本论文可能成为这一研究领域的里程碑式的论文。”

Dr Eddy Rubin, Director of the U.S. Department of Energy Joint Genome Institute and Director of the Genomics Division at Lawrence Berkeley National Laboratory in Berkeley added, "This study using a comparative genomics strategy discovered important human specific properties of the regulatory network in human ES cells. This information is significant and should contribute to helping move the regenerative medicine field forward."

美国能源部联合基因组研究所所长、劳伦期•伯克利国家实验室伯克利实验分室基因组学部主任埃迪•拉宾(Eddy Rubin)博士补充说:“这个运用了比较基因组学策略的研究在人类胚胎干细胞(ES细胞)中发现了重要的人类特异性属性。该信息意义重大,应该有助于推进再生医学领域的发展并作出贡献。”

(Docofsoul 译于2010-09-14)


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Docofsoul

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个人资料 发短消息 2010-09-14 23:15
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3 导读:六十年前, 生理学与医学诺贝尔奖获得者芭芭拉•麦克林托克提出了在当时可谓石破天惊的理论推测:转座因子,即可移动的遗传物质(DNA)片段(比如说病毒序列),一旦插入基因组,就能成为影响基因调节的“控制因子”。六十年后,科学家们凭借全新的测序技术,通过电脑计算与实验设计双管齐下的非凡策略,终于印证了这一假说。这一成就对基因组学的发展以及再生医学临床化都将有积极的意义。

—— Docofsoul

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共有1条评论
1   [DrNewbie 于 2010-11-21 02:20:56 提到] [FROM: 98.]


Medi is back to its glory days in the absence of the obnoxious barking from the MadDog!!! Does anyone think there are some indispensable tips he actually learned from his private club? His club is such a joke.

I cant believe this kind of idiot exists in this world. Two words to describe this piece of human trash: shameless and stupid. USMedEdu, aka, MadDog, put the following thread in his blog and bold it in the front page. Yet, clearly, the answers he endorsed are the worst answers. The answer I provided got a nod from an English forum. Does he understand the concept of shame or logic? Not only he speaks/writes shitty English, he cant think straight either. And now he wants to drag everyone down with him. PITY! What kind of dumb ass slaps his own face in public? What a joke!
无极: 住院医生面试碰到的尴尬事( 力刀评注推荐及麦地网友讨论)

Doc has successfully sabotaged a good learning club. At the peak, the pre_resident_english_corner had over 95 members and hundreds of posts. Congratulations! I will jot down a few expressions and new words here and there for myself mostly. Sorry friends. I let you down. You guys are the only losers as the bystanders caught in the cross-fire between the two warring parties. Ironically, both of the two people at war came out as the only two 'winners'. Doc can claim his star power. I can focus on my study.

Clearly, this USMedEd cant think straight anymore at his age. To give his credit where credit is due, he is still good at copying/pasting of the posts written by others. Follow his rubbish advice at your peril. We can write a laundry list of his shitty advice. This is a guy who cant keep his job for long. I am still wondering why he had to side with the losing party in a political struggle at work as he put it in one of his posts. Most people would not get involved. Only confrontational and aggressive douche bags would go for it. They can be burned again and again and are still clueless as it is just bad luck and not their faults whatsoever.

It is not just me saying this. Read this:
[snowfox01 于 2010-11-02 17:25:32 提到] [FROM: 134.74.]
Dr.Newbie:Please be nice to this old man。
难道你没看见你把他生命中唯一的支柱----收集各种贴子,然后大言不惭的copy&post 到自己的网页。给打碎了。同时,他在麦地的“一手遮天” 用那二十年前的狗屁经验来误导新人,也让你给揭穿了。我能理解他的恼羞成怒。他也没几天了,就让他乐呵乐呵吧。

There is no doubt that my English is better than anyone else including Eric and that lumcsomething. I was actually touched by the applications to my club every day during this short time. While I am still trying to figuring out the best way to get people energized and the best way to help, it went kaboom. It is such a pity. Just imagine how much more I can help once I get into residency and finish it.

I am sorry it has gone this far. This dokknife has been attacking me relentlessly on anything I write for no good reason. I have to stand up for myself. He said it himself: He does not care whether I am right or wrong. So he makes it personal. Now, he got it. It is personal between him and me.

Feel free to say hello to me at your leisure. I dont have time to play with this piece of human trash and a sadist who excels in copying/pasting and who cant keep his jobs for long. Let him have his schadenfreude from how the ex-boss who canned him got divorced. Let him brag how successful he is in dismantling a learning place.

Lets watch which comes first: I get into residency or he got fired.

并爱好给人改错的蠢人的发言更充分地反映出其愚蠢和无知,她的所谓良好英语在她的愚蠢脑袋支配下成了砸她自己脚的石头。此人在麦地已经贩卖了无数的垃圾和错误得东西。我实在无法忍受这种蠢人无休无止地误导CMG.
-----Doc.
乌龟总是对眼王八,狼和狈总是为奸。
你和这个混球对上眼很正常。装糊涂装黄花大姑娘真有坐台小姐得姿态嘛。
-----Doc
你这WSN还真是咸吃萝卜蛋操心.
-----Doc
耗子乌龟王八苍蝇和垃圾.
-----Doc
狗屁不懂的蠢货,愚蠢跳粱BSO牛Xer还真是第一次出现。各位可以饱眼福了.
-----Doc
换王八壳子来口水的蠢货的蠢言蠢语愚不可及的暴露,她懂个狗P.
-----Doc
我当我的医生,真也是撑了的,给你这号250上课来了还指望你懂人话呢。俺还真不如去打兔子大雁打网球去了。你这号真是纯粹的生物WSN德性。
-----Doc
向你这号又哭穷和没地方找饭碗,还不愿去换个活法,才真让人烦的WSN德性呢!真该去CT或MRI了!
-----Doc
你丫的又该酸掉几个牙?尔等难怪人家说生物萎缩,真TMD不亏!
-----Doc
说你等装B萎缩吧,你还扭扭捏捏跟坐台的要装黄花大姑娘似的。想来邪的,俺不是
不会更不是没掐过。
-----Doc



You all think this is acceptable and a good presentation of character? What I said back is half what he has said to me. Every time I make an apology to him, I got spit-at-your-face response in return.

It is a pity to see a good club falling apart. At least I have the decency not to use my club as a venting joint. I am sorry that you are put between a rock and a hard place. I will understand if you have to quit the club. You are always welcome to use a majia and check us out. This club is not intended as a base for isolation or a launch pad for personal attacks. It is a place purely devoted to English skills. It is a really a shame that Doc would pull people out of a club just to make people take sides. I dont see how he sabotages an educational club would help his cause. Yes, he has succeeded in pulling 20 people out of my English club and he can claim it is a win-lose-lose situation for himself. But ultimately, it is a lose-lose-lose situation.


1 [DrNewbie 于 2010-11-08 22:03:16 提到][删除][修改] [FROM: 98.119.]
Do you have any traces of honor and integrity left in your body? What a piece of human trash. How hard is it to admit you were wrong? What a stupid, arrogant, crazy bitch. Your bark is worse than your bite. Pathetic loser. You promote a 'clean' medi. Yet, you started a club for the sole purpose of shitting in it.You are a disgrace to the human race.

Read this and you know how it started and what I said is exactly what Doc said to me in English. And he said it a dozen of times to me.

http://www.mitbbs.com/pc/pccon_7773_142905.html

1 [DrNewbie 于 2010-11-08 23:51:41 提到][删除][修改]

What I said is half of what you have said to me. What are you whining about? Be a man. Fight fair and square. Dont just shit in your own compound. You thought you will get sympathy from your followers? What you get is despise from your sympathizers.

2 [DrNewbie 于 2010-11-08 23:05:40 提到][删除][修改]
Clearly you have no comprehension of English. You have a low EQ. You are clueless here. Why dont you shut your mouth? Be a man and make an apology to me. I have apologized to you a dozen of times. What I get is spit-at-my-face every time in return. Do you have any integrity and honor left in u?

3. All said and done. You truly think you gain the upper hand by locking me outside? You truly think I will lose without getting into your club? Seriously, dont flatter yourself. I have connections to match into a good University program already. I personally know a couple of Chinese PDs and American PDs. Give me a break already.

Lexian (蒙古大夫) 于 (Thu Feb 11 12:11:31 2010, 美东) 提到:

I think you are pointing this to me. I can honestly tell you i don't have
any MaJia. never had and never will. I don't hold personal grudge against
anybody, especially this one. it's not worth my time.

However, i think you are biased by assuming people who are against tyranny
and unjust are against anyone specifically. I can only speak for myself
because i really don't know anyone else that got into similar kind of
argument with him. I have no intention of "打击正在做的人", but that doesn't
mean i won't say "WAIT A MINUTE, THIS IS NO RIGHT" when i see posts like
the one i respond to.

As i have stated long long time ago, CMG's history in this country are short
. Our mission goes far beyond getting into residency. A public forum that
attracts examiners, residents and PRACTICING PHYSICIANS is very important in
exchange information and experience. I just can't help laughing when i see
some guy who happen to got into residency wants to "harmonize" the forum. I
can honestly say the game just starts AFTER you graduate. Please refrain
from your "GOD" like euphoria of getting into the residency,

发信人: amanda12 (digest08), 信区: Biology
标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Fri Oct 22 08:01:00 2010, 美东)

我想说的是,作为一名医生,应该有更高的道德标准,宽容心和同情心,医生是和人打
交道的职业,会碰到各种各样人,首先我们想到的是他是我的病人,不管他是杀人犯还
是某某人大代表,穷人和富人,都要统一对待。不会因为别人过激言语或者什么而去计
较,要这有宽容心,能站在他人的角度去理解对方,这才会得到对方的信任。不要动不
动就对别人丢WSN。。。你只会显出你心胸狭隘,既与你的医生身份不配,也会让人鄙
视!以前看过你在丁香园的帖子,貌似你没完成专科移植是因为2老美因为你手术做得
好排挤你。
别人稍与你意见不同就恶言相向,言辞激烈,这是你说服别人的办法吗?是从一名医生
口里出来的话吗?要得到别人的尊重和赞美,同时需要你在本专业的优秀和个人魅力,
一言一行感动别人,取得别人信任,医生更是如此。尊重别人的同时,别人才会尊重你
也许力刀的初衷是好的,但是从他的言语看来,更让人觉得是个暴发户。。。。

发信人: Viky (转身插口袋), 信区: Biology
标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Thu Oct 21 20:03:42 2010, 美东)

只有250才会在网上显摆自己的薪水,当年这厮也显摆自己在osu的薪水,现在总算得到
头的"亲徕"了

发信人: redasuka (EVA-02), 信区: Biology
标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Fri Oct 22 03:59:15 2010, 美东)

没啥奇怪的,考版的那些人本身就花了比一般PhD多得多的时间和精力,当然希望能换
来一点优越感。何况不少大陆的MD最后在北美都是在做内科儿科什么的,每天工作也很
压抑,只好靠贬低别人获得一点心理安慰啦。

说实话,我在这边认识的混得好的中国MD还没有Bio/BME的faculty比率高,绝大多数30
-40岁的Attending还是处在天天接待黑哥们的境界,收入税前也就100k多点。真正混得
好、拿大钱的的MD,那是少数,而且基本都是白人。

不是说老中没有MD混得好的,只不过那些混得好的是人家真牛,在哪一行能牛起来。至
于只是为了混碗饭吃的,还是要多掂量几下自己折腾的起不。

发信人: Kiwixi (kiwi), 信区: Biology
标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Fri Oct 22 04:05:37 2010, 美东)

这斯绝对是凭着CMG来美国弄了一个博后的位子。可想而知,实验室做的一塌糊涂,老
板天天骂,连老婆也看不起,留空就跑到楼外面捡烟屁股抽。
然后就考了版,摇身一变就成了医生。

薪水涨了,心魔却去不了,跟同行比,口音重,年龄大,还是来这里显摆,
打着普渡众生的幌子,年复一年贴几个链接,满足自己的虚荣心。本来考版当医生就是
一个职业,非要搞成一个崇拜一个图腾,入门的教众齐颂文成武德哈里路亚癫狂不已。

你那几个链接还是自己留在记事本里吧。不需要你这样自上而下的伪善。

最后送你几句话,真的是为你好,淡定一些,从容一些,都快半百的人了。

他人骑大马,我独跨驴子,回顾担柴汉,心下较些子。
发信人: snowfox01 (白面狐), 信区: Biology
标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Fri Oct 22 14:16:58 2010, 美东)


我可以理解许多国人为五斗米折腰,想得到需要的信息。但是十年前的经验今天不一定
适用了。而且他像传销一样给许多老CMG False hope,一些老毕业生像打了鸡血一样跟
随他, 做了不且实际的选择, 也不知害了多少家庭。精神病人很可怕, 若他再领着
一帮人, 这帮人真是可怜。 take a look:

发信人: USMedEdu (US_CMGs), 信区: MedicalCareer
标 题: Re: 考版俱乐部(Pre_Resident_Club)成立!
发信站: BBS 未名空间站 (Fri Oct 22 10:45:26 2010, 美东)

click the link and go to club site to apply. Once you apply there, your ID
will be in waiting list for approval.

发信人: lostknife (麦地俯卧撑), 信区: MedicalCareer
标 题: Re: 考版俱乐部(Pre_Resident_Club)成立!
发信站: BBS 未名空间站 (Fri Oct 22 13:03:31 2010, 美东)

I fully support the idea and be happy to join.
But it shows:抱歉, 缺少参数, 加入俱乐部失败!
please advise what I should do to join the club.
Thanks.

发信人: lostknife (麦地俯卧撑), 信区: MedicalCareer
标 题: Re: 考版俱乐部(Pre_Resident_Club)成立!
发信站: BBS 未名空间站 (Fri Oct 22 13:05:16 2010, 美东)

By the way, I am a good-egg who hate the bad-egg!

【 在 meigui0714 (rose) 的大作中提到: 】
: 谢谢你的(USMedEdu)忠告。"听人劝,吃饱饭"。我会好自为之的。
: 但是,一个好的论坛氛围,需要大家的努力。我们做为老生,应该已实事求是的态度引: 导后生们走好,走稳自己的路。人生只有一次,尤其是毕业时间长,没有经历过美国正: 规教育的老生们,我们已经没有"试验”"尝试"的时间了。三思而后行, 一旦做出了决: 定,就不要犹豫,大刀阔fu, 勇往直前。
: 尊敬的老前辈,这里是公共论坛,不是你个人的博客。老了,不但应该"自尊,自爱",: 更要尊重他人的不同看法,意见,这样才能赢得更多的尊敬。“唯我独尊”“我是老: 大我怕谁”的心态是万万要不得的。这里不是"黑色会”,这里不需要“教父”!: 读者的眼睛是雪亮的,这个论坛的读者们都是受过高等教育的,相信他们清楚,谁更浅薄。


发信人: Oncogene (何时问天), 信区: Biology
标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Wed Oct 20 18:22:24 2010, 美东)

不要再“尔等,尔等”的了,让人看了烦。身边考版做医生的太多太多了,也没见几个
这么嚣张的。

我不相信这世界有啥救世主。试图做救世主的,脑袋真的需要MRI啦

发信人: yoyoch (yoyoch), 信区: Biology
标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Wed Oct 20 23:39:59 2010, 美东)

素质,素质。
风度,风度。

发信人: KeeVan (Kevin), 信区: Biology
标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Wed Oct 20 23:57:59 2010, 美东)

你说你瞎急啥。就是因为你没说赚多少钱,但是又说生物wsn泛酸,我没看明白人家为什么泛酸。讨论讨论问题不行啊,你这素质也太低贱了,我看还是别行医了

发信人: pigsun (屁哥~~大圣教候补二师兄), 信区: Biology
标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Thu Oct 21 01:07:33 2010, 美东)

散了吧,老刀确实不是来劝退的,也不是故意显摆,其实,他就是专门来找抽的...
--
哈,说出来心里舒服多哩

发信人: Oncogene (何时问天), 信区: Biology
标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Thu Oct 21 01:25:47 2010, 美东)

我都懒得抽她。这么大年纪了,好好说话会死啊??

发信人: Oncogene (何时问天), 信区: Biology
标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Thu Oct 21 01:33:02 2010, 美东)

最后回你一贴吧:
你还是真是个幻想狂人唉。还有吧,就你这口气和心态,现实中能过得舒服还真是见了鬼了。算了,刚才说了,说你也是白说。你还是好好找工作吧,最好找个$1million/yr的,可千万别把自己的高等生活给葬送了呀,走到今天(昨天)也怪不容易的。。。

发信人: Oncogene (何时问天), 信区: Biology
标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Thu Oct 21 01:48:19 2010, 美东)

^_^,你这风向转的还真快

靠骂别人或者教训别人是不可能有效传播自己的信息的。就这种性格,他下个工作也
干不久,哪个上下级能受得了这种人啊。好象别人没见过钱没见过医生没见过成功
人士一样,一副高高在上的架势,受不了。

发信人: newlily (lily), 信区: Biology
标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Thu Oct 21 09:18:43 2010, 美东)

老刀,本来我是挺尊敬您的,想从您的帖子学点东西,结果谩骂满篇,没有一点有用的东西。

发信人: Viky (转身插口袋), 信区: Biology
标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Thu Oct 21 11:19:53 2010, 美东)

这厮两次被fired了,还特喜欢到处指手画脚

标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Thu Oct 21 14:10:26 2010, 美东)

1.相信大家都有北美工作经验,新chair来了,就辞掉所有的人,可能吗?道是有可能
。。。。,

2.“加拿大拿的工资比美国大学薪水要高一倍可工
: 作量才是美国的1/4(其实俺每天1小时就干完了!)” 这种事情只会发生在社会主义
中国。 加拿大? 你可别弄的跟唐骏似的。
3.你是为了祖国加拿大的医学发展,毅然决然放弃了美国的高薪
工作,回到了加拿大了吧。

发信人: Viky (转身插口袋), 信区: Biology
标 题: Re: 给生物同学们: dojo: 走进美国医学院之旅
发信站: BBS 未名空间站 (Thu Oct 21 11:31:42 2010, 美东)

正常个p, 被fired了就是被fired, 没见过这么不要脸的


【 在 USMedEdu (US_CMGs) 的大作中提到: 】
: 窝草! 俺自己在网上说过,还怕你来拾俺的鞋后跟泥巴砸俺不成?
: 被新chair辞了,换朝换臣找新工作在北美不是常事吗?哪个医生老死在一个单位的?
: 俺要再显摆一把告诉你俺现在在加拿大拿的工资比美国大学薪水要高一倍可工
: 作量才是美国的1/4(其实俺每天1小时就干完了!)你丫的又该酸掉几个牙?
: 尔等难怪人家说生物萎缩,真TMD不亏!




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共有2条评论
1 [Viky 于 2010-11-12 09:42:23 提到][删除] [FROM: 152.11.]
劝 lz别跟那个老250浪费时间了。。
 
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